Huberman Lab
Mastering Nutrition
Morning Air
Diet Science
Mind Pump: Raw Fitness Truth

Glycine

5 episodes B

Episodes covering glycine — protocols, research, and expert discussions.

View Glycine →

Simple amino acid that improves sleep quality, supports collagen synthesis, and may promote longevity

Glycine is one of the simplest, cheapest, and most underrated sleep supplements. Taking 3g before bed consistently improves subjective sleep quality, reduces time to fall asleep, and helps you wake feeling more refreshed, with virtually no side effects.

The mechanism is elegant: glycine lowers core body temperature, which is a key signal for sleep initiation. It also acts on NMDA receptors in the brain's sleep centers and may increase serotonin availability.

Beyond sleep, glycine is a building block for collagen (every third amino acid), a precursor to glutathione (master antioxidant), and has been shown to extend lifespan in animal models by mimicking methionine restriction.

At $0.10-0.30/day with essentially no downside, glycine is worth trying for anyone with sleep issues or interested in longevity. It's the rare supplement where the risk/reward ratio is strongly favorable.

Science & Mechanisms

How Glycine Improves Sleep:

  1. Thermoregulation - Glycine causes peripheral vasodilation, which dissipates heat from the body's core. This drop in core temperature is a critical signal for sleep onset. Studies show glycine reduces core body temperature by ~0.5°C.
  1. NMDA Receptor Modulation - Glycine acts on NMDA receptors in the suprachiasmatic nucleus (the brain's master clock), promoting sleep through neural mechanisms.
  1. Serotonin Pathway - Glycine may increase serotonin availability in the brain, supporting both sleep and mood.

Clinical Evidence:

  • Yamadera et al. (2007): 3g glycine improved subjective sleep quality and reduced daytime sleepiness in people with sleep complaints
  • Bannai et al. (2012): 3g glycine improved next-day cognitive performance in sleep-restricted subjects
  • Kawai et al. (2015): Confirmed NMDA receptor mechanism in suprachiasmatic nucleus

Longevity Mechanisms:

  • Methionine restriction mimetic - Glycine may counteract excess methionine (common in high-protein diets), activating longevity pathways
  • Glutathione precursor - Combined with NAC (as "GlyNAC"), supports master antioxidant synthesis
  • Collagen synthesis - Glycine is 33% of collagen; supplementation supports structural protein maintenance
  • Autophagy activation - May promote cellular cleanup mechanisms

Animal Evidence:

  • Glycine extended lifespan by 4-6% in genetically diverse mice
  • GlyNAC extended lifespan by 24% in mice

Episodes

1
Huberman Lab
Peptide & Hormone Therapies for Health, Performance & Longevity | Dr. Craig Koniver
Huberman Lab Dr. Craig Koniver 2024-10-07

Andrew Huberman hosts Dr. Craig Koniver, a board-certified physician specializing in performance medicine, for a deep dive into the therapeutic use of peptides and hormones for ...

2
Mastering Nutrition
Startled?! Try Glycine!
Mastering Nutrition Chris Masterjohn 2023-10-28

Chris Masterjohn explores the calming effects of glycine supplementation, particularly its role in reducing the startle response and supporting nervous system regulation. He rev...

3
Morning Air
Strength in Suffering/ Glycine Miracle
Morning Air Joel Brind 2025-09-02

Yale-educated biochemist Dr. Joel Brind joins Morning Air to explain his theory that chronic inflammation is driven by widespread glycine deficiency rather than being a normal r...

4
Diet Science
The Sweet Benefits of Glycine
Diet Science 2023-09-18

Nutritionist Dee McCaffrey covers glycine's wide-ranging health benefits on this episode of Diet Science. She explains that glycine serves as a building block for glutathione, t...

5
Mind Pump: Raw Fitness Truth
2650: Top 7 Underrated Supplements
Mind Pump: Raw Fitness Truth 2025-07-28

Sal DiStefano, Adam Schaefer, and Justin Andrews rank their top seven underrated supplements that most fitness enthusiasts overlook. The list covers vitamin D (cheap, widely def...

Related Research

The effect of glycine administration on the characteristics of physiological systems in human adults: A systematic review.
Soh J, Raventhiran S, Lee JH, et al. (2024)
Systematic review of 50 human studies found glycine most consistently benefits the nervous system, improving sleep quality in healthy adults and psychiatric symptoms in clinical populations.
An Update of the Promise of Glycine Supplementation for Enhancing Physical Performance and Recovery.
Ramos-Jiménez A, Hernández-Torres RP, Hernández-Ontiveros DA, et al. (2024)
Glycine shows promise for physical performance and recovery through modulation of oxidative stress, inflammation, and muscle protein synthesis, but most evidence comes from animal studies and human ergogenic evidence remains insufficient.
Glycine: The Smallest Anti-Inflammatory Micronutrient.
Aguayo-Cerón KA, Sánchez-Muñoz F, Gutierrez-Rojas RA, et al. (2023)
Glycine exerts broad anti-inflammatory effects by inhibiting NF-κB signaling, reducing pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), increasing anti-inflammatory IL-10, and suppressing cell death pathways across multiple tissue types.
Glycine and N-acetylcysteine (GlyNAC) supplementation in older adults improves glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, insulin resistance, endothelial dysfunction, genotoxicity, muscle strength, and cognition: Results of a pilot clinical trial
Sekhar RV, et al. (2022)
GlyNAC supplementation in older adults corrected glutathione deficiency and improved multiple hallmarks of aging.
The sleep-promoting and hypothermic effects of glycine are mediated by NMDA receptors in the suprachiasmatic nucleus
Kawai N, Sakai N, Okuro M, et al. (2015)
Glycine promotes sleep by acting on NMDA receptors in the suprachiasmatic nucleus, causing peripheral vasodilation and core temperature drop.
The effects of glycine on subjective daytime performance in partially sleep-restricted healthy volunteers
Bannai M, Kawai N, Ono K, Nakahara K, Murakami N (2012)
3g glycine improved next-day cognitive performance and reduced fatigue in sleep-restricted subjects.